METHOD FOR ASSESSING THE OROPHARYNGEAL DYSBIOSIS IN CHILDREN Russian patent published in 2021 - IPC A61B5/00 G01N33/487 G01N30/12 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to clinical laboratory diagnostics, and can be used for evaluating the oropharyngeal dysbiosis in children. Content of acetic, propionic, isobutyric, butyric and caproic acids is determined in the saliva of a child by gas-liquid chromatography. Then, using classification equations of linear discriminant analysis, the value of diagnostic coefficients evaluating the dysbiosis state is calculated by formulas F₁=12210.91*C2+11890.28*C3+13156.14*iC4+12226.24*C4+42514.51*C6-6025.33, F₂=12015.23*C2+11738.47*C3+12952.92*iC4+12062.53*C4+41838.19*C6-5846.01, where F₁ is the diagnostic coefficient, at the maximum value of which the oropharyngeal dysbiosis is established; F₂ is the diagnostic coefficient, at the maximum value of which the oropharyngeal dysbiosis is not established; C2 is relative concentration of acetic acid; C3 is relative concentration of propionic acid; iC4 is isobutyric acid relative concentration; C4 is the relative concentration of butyric acid; C6 is relative concentration of caproic acid. If F₁ is more than F₂, oropharyngeal dysbiosis is established, and if F₁ is less than F₂, absence of oropharyngeal dysbiosis is established.

EFFECT: method provides fast and reliable assessment of oropharyngeal dysbiosis in children by identifying a specific ratio of concentrations of single-chain fatty acids in saliva, that allows to take into account changes in all loci of oropharyngeal biotope and change of anaerobic part of microbiocenosis.

1 cl, 2 ex