TEST SYSTEM FOR IDENTIFICATION OF BEAR TISSUE (URSUS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS Russian patent published in 2020 - IPC C12Q1/68 

Abstract RU 2726429 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identification of DNA of bear tissue (Ursus) in dry fodder and meat semi-products, comprising a buffer for carrying out a polymerase chain reaction, a mixture for carrying out thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for the animal genome region and for the internal control sample; mixture of enzymes from DNA polymerase with enzyme inhibiting antibodies, TAQ POLYMERASE, an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×103 phagic particles per 1 mcl, a negative control sample which is a mixture of recombinant plasmid DNA containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome.

EFFECT: invention can be used to expand functional capabilities and increase accuracy of identification of species identity, simplify sample preparation process.

1 cl, 5 tbl

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Authors

Chernykh Oleg Yurevich

Bannov Vasilij Aleksandrovich

Kotelnikova Aleksandra Andreevna

Malyshev Denis Vladislavovich

Chernykh Vladimir Olegovich

Lysenko Aleksandr Anatolievich

Koshchaev Andrej Georgievich

Krivonos Roman Anatolevich

Drobin Yurij Dmitrievich

Luneva Albina Vladimirovna

Nesterenko Anton Alekseevich

Inyukina Tatyana Andreevna

Sharavev Pavel Viktorovich

Yuldashbaev Yusupzhan Artykovich

Zabashta Nikolaj Nikolaevich

Dates

2020-07-14Published

2019-10-16Filed