FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identification of DNA of bear tissue (Ursus) in dry fodder and meat semi-products, comprising a buffer for carrying out a polymerase chain reaction, a mixture for carrying out thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for the animal genome region and for the internal control sample; mixture of enzymes from DNA polymerase with enzyme inhibiting antibodies, TAQ POLYMERASE, an internal control sample in the form of a bacteriophage T4 suspension with concentration of 5×103 phagic particles per 1 mcl, a negative control sample which is a mixture of recombinant plasmid DNA containing a fragment of the animal's genome and a fragment of the bacteriophage T4 genome.
EFFECT: invention can be used to expand functional capabilities and increase accuracy of identification of species identity, simplify sample preparation process.
1 cl, 5 tbl
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR IDENTIFICATION OF BEAR TISSUE (URSUS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2726427C1 |
METHOD FOR IDENTIFICATION OF DOMESTIC CAT TISSUE DNA (FELIS SILVESTRIS CATUS) IN DRY FODDERS AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2728662C1 |
TEST SYSTEM FOR IDENTIFICATION OF COMMON HEDGEHOG (ERINACEUS EUROPAEUS) TISSUE DNA IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2725215C1 |
TEST SYSTEM FOR DETERMINATION OF WOODPECKER (PICIDAE) DNA IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2725216C1 |
METHOD FOR IDENTIFICATION OF COMMON HEDGEHOG (ERINACEUS EUROPAEUS) DNA TISSUE IN DRY FODDER AND SEMI-FINISHED MEAT | 2019 |
|
RU2726433C1 |
TEST SYSTEM FOR IDENTIFICATION OF DNA OF DOMESTIC CAT TISSUE (FELIS SILVESTRIS CATUS) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2728639C1 |
METHOD FOR IDENTIFICATION OF RAT AND MOUSE TISSUE SPECIES IN DRY FEED AND SEMI-FENISHED MEAT PRODUCTS | 2019 |
|
RU2742952C1 |
METHOD FOR WOODPECKER (PICIDAE) TISSUE DNA DETERMINATION IN DRY FEED AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2714287C1 |
TEST SYSTEM FOR IDENTIFICATION OF DNA TISSUES OF RATS AND MICE IN DRY FODDER AND MEAT SEMI-PRODUCTS | 2019 |
|
RU2725539C1 |
METHOD FOR IDENTIFICATION OF DNA TISSUE OF COMMON QUAIL (COTURNIX COTURNIX) IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS | 2019 |
|
RU2734035C1 |
Authors
Dates
2020-07-14—Published
2019-10-16—Filed