TEST SYSTEM FOR DETERMINATION OF WOODPECKER (PICIDAE) DNA IN DRY FODDER AND SEMI-FINISHED MEAT PRODUCTS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Invention is a test system for identifying DNA of woodpecker tissue (Picidae) in dry fodder and meat semi-products, comprising a buffer for carrying out a polymerase chain reaction, a mixture for carrying out thereof, consisting of deoxynucleoside triphosphates, oligonucleotide primers and fluorescent probes specific for the animal genome region and for the internal control sample; a mixture of enzymes from DNA polymerase with antibodies inhibiting enzyme activity, TAQ POLYMERASE, an internal control sample in the form of suspension of bacteriophage T4 with concentration of 5×10³ phage particles per 1 mcl, a negative control sample, which is a mixture of recombinant plasmid DNA containing a fragment of an animal's genome and a fragment of the bacteriophage T4 genome with a nucleotide sequence, taken in volume ratio of 1:1, according to the invention, phagolysate of bacteriophage T4 is used for internal control sample, and for positive control sample fragments of genomes of native bacteriophage T4 and woodpecker (Picidae) with the following nucleotide sequence are used.

EFFECT: invention widens functional capabilities and accuracy of identification of species identity, simplifies the preparation process.

1 cl, 5 tbl, 1 dwg