FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce recombinant proinsulin human glargine. Recombinant proinsulin is obtained in a hybrid polypeptide, in which the human HGS protein fragment, the tyrosine kinase substrate, is linked through peptide linker with sequence GlyHis6GlySerArg with amino acid sequence proinsulin of human glargines, with arginine as site of proteolysis between C-peptide and chain A. For this purpose, recombinant plasmid DNA pF644 and strain-producer Escherichia coli BL21/pF644 are used.
EFFECT: invention provides synthesis of a hybrid polypeptide with an expression level of not less than 4 % of the crude weight of the cell biomass, enables increasing the percentage of insulin glargine in the hybrid protein to 49_5 % and improving the purification efficiency of the end product by eliminating the closely related Arg0-A-glargine.
3 cl, 6 dwg, 6 ex
| Title |
Year |
Author |
Number |
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