FIELD: genetic engineering, proteins.
SUBSTANCE: recombinant plasmid DNA pHINS05 comprises gene encoding hybrid protein consisting of N-terminal fragment of human gamma-interferon, peptide linker His4GlySerArg and human proinsulin. E. coli cells are transformed with recombinant plasmid DNA pHINS05 and the strain E. coli JM109/pHINS05 is obtained that is a producer of hybrid protein with human proinsulin. Invention provides increasing yield of recombinant human proinsulin and to reduce cost of the technological process in preparing human insulin.
EFFECT: valuable biological properties of plasmid DNA and strain.
4 cl, 2 dwg, 4 ex
| Title |
Year |
Author |
Number |
| RECOMBINANT PLASMID DNA PPINS07 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Vul'Fson A.N.
- Kostromina T.I.
- Miroshnikov A.I.
- Gavrikov V.G.
- Kalinin Ju.T.
- Stepanov A.V.
|
RU2144957C1 |
| RECOMBINANT PLASMID DNA pHI03, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA pHI03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHI03-PRODUCENT OF HYBRID PROTEIN-PRECURSOR OF HUMAN INSULIN |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2515061C1 |
| RECOMBINANT PLASMID DNA pHILP07 ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHILP07, AND STRAIN OF BACTERIA Escherichia coli JM109/pHILP07 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Lispro |
2013 |
- Shmatchenko Vadim Vasil'Evich
|
RU2514480C1 |
| RECOMBINANT PLASMID DNA pHINS11 CODING HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, Escherichia coli CELL, TRANSFORMED WITH RECOMBINANT PLASMID DNA pHINS11, Escherichia coli BACTERIA STRAIN JM109/pHINS11 - PRODUCER OF HYBRID PROTEIN-HUMAN INSULIN PRECURSOR, AND METHOD OF OBTAINING HUMAN INSULIN |
2006 |
- Shmatchenko Vadim Vasil'Evich
- Shmatchenko Natal'Ja Anatol'Evna
- Bajdus' Aleksandr Nikolaevich
- Kuptsov Vasilij Nikolaevich
- Nozdrin Viktor Nikolaevich
- Stepanov Aleksej Vjacheslavovich
|
RU2354702C2 |
| RECOMBINANT PLASMID DNA pHIG05, CODING HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine, CELL Escherichia coli, TRANSFORMED BY RECOMBINANT PLASMID DNA PHIG05, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIG05-PRODUCENT OF HYBRID PROTEIN WITH HUMAN PROINSULIN Glargine |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2515115C1 |
| RECOMBINANT PLASMID DNA pHIASP03, ENCODING HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart, CELL Escherichia coli TRANSFORMED BY RECOMBINANT PLASMID DNA pHIAsp03, AND STRAIN OF BACTERIA Escherichia coli JM109/pHIAsp03 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN Aspart |
2013 |
- Shmatchenko Vadim Vasil'Evich
- Sadgjan Armen Sergeevich
|
RU2514578C1 |
| RECOMBINANT PLASMID DNA PF265 CODING HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN, AND BACTERIAL STRAIN ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2728611C1 |
| RECOMBINANT PLASMID DNA PPINS16 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN, RECOMBINANT PLASMID DNA PPINS25 ENCODING FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN AND STRAIN OF BACTERIUM ESCHERICHIA COLI - PRODUCER OF FUSED POLYPEPTIDE CONTAINING HUMAN PROINSULIN |
1999 |
- Korobko V.G.
- Boldyreva E.F.
- Shingarova L.N.
- Miroshnikov A.I.
- Gavrikov V.G.
- Stepanov A.V.
- Kalinin Ju.T.
|
RU2143493C1 |
| RECOMBINANT PLASMID DNA PF267 CODING HYBRID POLYPEPTIDE CONTAINING PROINSULIN LISPRUM, AND A STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF HYBRID POLYPEPTIDE CONTAINING PROINSULIN LISPRO |
2019 |
- Latypov Vitaliy Felixovich
- Kornakov Igor Aleksandrovich
- Robustova Svetlana Eduardovna
- Khomutova Oksana Sergeevna
- Rodionov Petr Petrovich
|
RU2729357C1 |
| STRAIN OF BACTERIA Escherichia coli JM109/pHINS21 - PRODUCER OF HYBRID PROTEIN WITH HUMAN PROINSULIN AND METHOD FOR PRODUCTION OF HUMAN PROINSULIN |
2007 |
- Rodionov Petr Ivanovich
- Rodionov Petr Petrovich
- Shmatchenko Vadim Vasil'Evich
- Stepanov Aleksej Vjacheslavovich
- Bajdus' Aleksandr Nikolaevich
- Shmatchenko Natal'Ja Anatol'Evna
- Gorkun Taras Alekseevich
|
RU2376368C2 |
