IMPROVED DETECTION OF SHORT HOMOPOLYMERIC REPEATS Russian patent published in 2020 - IPC C12Q1/6858 C12Q1/6886 C12N15/11 

FIELD: biotechnology.

SUBSTANCE: disclosed is a method and a kit for detecting changes in the number of nucleotides present in the target sequence of nucleotide repeats, the length of which is 12 bp or less. Method involves obtaining amplicons, heating amplicons in the presence of a signal-generating oligonucleotide probe and establishing the homopolymer reiterations of the number of nucleotides present in the target sequence based on the melting curve. Kit contains an oligonucleotide probe of the "molecular beacon" type and having 3'-5' exonuclease activity of the polymerase. Oligonucleotide probe contains a sequence which is capable of hybridizing with a sequence containing homologous duplicate repeats by succession.

EFFECT: invention provides high-sensitivity and multiplexing, fully automated method of detecting changes in the number of nucleotides in short homopolymer microsatellites, which can be used by any standard thermal cycling device within the quantitative PCR.

19 cl, 5 dwg