NUCLEIC ACID PROBE AND A METHOD OF DETECTING GENOME FRAGMENTS Russian patent published in 2020 - IPC C12Q1/68 

FIELD: biotechnology.

SUBSTANCE: described is a method of processing a nucleic acid sample. In some versions of the invention, the method involves a) hybridization of a sample containing a target fragment to a nucleic acid probe, involving: i) a sequence located at the beginning and a sequence located at the end, wherein sequences located at the beginning and at the end are at the ends of the first oligonucleotide molecule; and ii) shunt sequence, which contains, in the following order: overlying flanking sequence, which is complementary to the sequence located at the beginning; a sequence complementary to the target, which is complementary to the target fragment; and an underlying flanking sequence, which is complementary to the sequence located at the end; as a result, a hybridization product is formed, in which the ends of the target fragment are adjacent for ligation to ends of sequences located at the beginning and at the end, in the first oligonucleotide molecule; and b) ligating the ends of the target fragment to the ends of the sequences located at the beginning and end of the first oligonucleotide molecule, resulting in an annular product, containing target fragment and sequences located at the beginning and at the end. Disclosed also are probes and kits for method implementation.

EFFECT: nucleic acid probe and a method of detecting genome fragments are provided.

37 cl, 10 dwg, 3 ex