METHOD OF AMPLIFICATION AND IDENTIFICATION OF NUCLEIC ACIDS Russian patent published in 2024 - IPC C12Q1/6855 

FIELD: biotechnology.

SUBSTANCE: method of obtaining labeled amplification fragments of a nucleic acid matrix is described. A nucleic acid template is provided. At least two oligonucleotide primers are annealed to a nucleic acid template, where at least one underlying primer is an elongation stopper. Elongation of at least two oligonucleotide primers is carried out in a template-specific manner. The elongation reaction stops when the elongation product reaches 5'-end of the nucleic acid template or oligonucleotide primer as a nucleic acid elongation stopper, hybridized on the nucleic acid template below the elongation product. A plurality of at least two adapter nucleic acids are provided, wherein the said plurality of adapters contain different identification sequences on their 5'-end, and these identification sequences do not hybridize with the oligonucleotide primer, which is an elongation stopper, or with the template. The adapter nucleic acid is ligated at its 5'-end with 3'-end of the elongation product, and for ligation, the elongation products are hybridized with the matrix, thereby obtaining a labeled amplification fragment. Also the following is described: a set containing at least two oligonucleotide primers capable of hybridizing with the template nucleic acid and triggering the elongation reaction on its 3'-end, where at least one primer is an elongation stopper, a plurality of at least two adapter nucleic acids, wherein the said plurality of adapters contain different identification sequences on their 5'-end, wherein such identification sequences do not hybridize with oligonucleotide primers, preferably where the adapter nucleic acid either binds and hybridizes, or does not bind and does not hybridize with the oligonucleotide primer, reverse transcriptase and oligonucleotide ligase.

EFFECT: invention expands the arsenal of agents for delivering nucleic acids.

15 cl, 5 dwg, 5 ex