FIELD: biotechnology.
SUBSTANCE: variant of phosphoribosyl pyrophosphate amidotransferase, where (1) amino acid in position 2 is replaced by methionine, (2) the amino acid at position 445 is replaced with arginine or (3) the amino acid in position 2 is replaced by methionine and the amino acid at position 445 is replaced by arginine from the N-terminus of the amino acid sequence SEQ ID NO: 2. Polynucleotide coding a version of phosphoribosyl pyrophosphate amidotransferase. Microorganism of the genus Corynebacterium producing a purine nucleotide, where the microorganism contains a variant of phosphoribosyl pyrophosphate amidotransferase. Method for producing a purine nucleotide involves a step for culturing said microorganism of the genus Corynebacterium in a medium.
EFFECT: group of inventions provides a variant of phosphoribosyl pyrophosphate amidotransferase for culturing a microorganism of the genus Corynebacterium for producing a purine nucleotide with high output.
7 cl, 10 tbl, 5 ex
