NOVEL PROMOTER AND METHOD OF PRODUCING PURINE NUCLEOTIDES USING SAME Russian patent published in 2020 - IPC C12N1/21 C12N15/77 C12P19/32 

FIELD: biotechnology.

SUBSTANCE: invention relates to a polynucleotide having promoter activity, a recombinant microorganism containing it, and a method of producing purine nucleotides using said microorganism. Disclosed is a polynucleotide having novel promoter activity, wherein in the polynucleotide sequence SEQ ID NO: 1 (1) 143^th nucleotide is replaced with thymine (T); (2) 189^th nucleotide is replaced with thymine (T); or (3) 143^th nucleotide is replaced with thymine (T) and 189^th nucleotide is replaced with thymine (T). Also disclosed is a recombinant microorganism of the genus Corynebacterium for producing purine nucleotides, containing said polynucleotide functionally related to a gene coding the target protein. Also disclosed is a method of producing purine nucleotides, involving cultivation of said recombinant microorganism of the genus Corynebacterium in a medium.

EFFECT: invention enables to obtain purine nucleotides with high output.

7 cl, 11 tbl, 5 ex