FIELD: medicine; biotechnology.
SUBSTANCE: invention refers to medical virology and biotechnology. Disclosed is a method for human culture rotavirus inactivation, consisting in that a human rotavirus suspension obtained by successive passages on a culture of inoculated cells of SPEV animals, triple freezing-thawing, centrifugation at 5,000 g for 30 minutes and control of cytopathogenic action on animal cell culture, centrifuged at 40,000 g for 5 minutes; obtained supernatant virus-containing liquid is placed in a tray of quartz glass with size of 20 × 200 mm, the cuvette is placed at distance of 1 mm from the llama surface of a bactericidal radiator OBN-75 UHL4 and exposed to ultraviolet radiation at wave length 253.7 nm for 5 minutes.
EFFECT: invention provides 100 % inactivation of infectious properties of rotavirus, does not change the protein structure and preserves its immunogenic properties.
1 cl, 2 dwg, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| Method of human culture rotavirus inactivation | 2020 |
|
RU2743300C1 |
| SORBED INACTIVATED VACCINE AGAINST CATTLE ROTAVIRAL INFECTION | 2005 |
|
RU2302258C1 |
| COMBINED EMULSION INACTIVATED VACCINE AGAINST ROTAVIRAL AND CORONAVIRAL CATTLE INFECTIONS | 2008 |
|
RU2366458C1 |
| ASSOCIATED INACTIVATED EMULSION BOVINE VIRAL DIARRHOEA, ROTAVIRUS AND CORONAVIRUS INFECTIONS VACCINE | 2012 |
|
RU2515058C1 |
| CATTLE ROTAVIRUS STRAIN FOR PRODUCTION OF DIAGNOSIS, VACCINE AND THERAPEUTIC PREPARATIONS | 2004 |
|
RU2264458C1 |
| TRANSMISSIBLE GASTROENTERITIS OF SWINE VIRUS STRAIN FOR PRODUCTION OF DIAGNOSIS AND VACCINE PREPARATIONS | 2004 |
|
RU2266326C1 |
| TRANSMISSIBLE GASTROENTERITIS OF SWINE VIRUS STRAIN FOR PRODUCTION OF DIAGNOSIS AND VACCINE PREPARATIONS | 2004 |
|
RU2266328C1 |
| TRANSMISSIBLE GASTROENTERITIS OF SWINE VIRUS STRAIN FOR PRODUCTION OF DIAGNOSIS AND VACCINE PREPARATIONS | 2004 |
|
RU2266327C1 |
| METHOD OF HUMAN ROTAVIRUS ADAPTATION TO GROWTH ON TRANSPLANTATED ANIMAL CELL CULTURES | 2018 |
|
RU2692604C1 |
| METHOD OF PREPARING VACCINE AGAINST PORCINE TRANSMISSIBLE GASTROENTERITIS AND A METHOD FOR PREVENTING PORCINE TRANSMISSIBLE GASTROENTERITIS | 2006 |
|
RU2306951C1 |
