FIELD: biotechnology, gene engineering, medicine, pharmaceutical industry.
SUBSTANCE: method production of preparative amounts of plasmid DNA from recombinant microorganism cells includes cultivation of recombinant cells in gas-vortex bioreactor, plasmid DNA isolation and centrifugal purification.
EFFECT: increased yield of target product.
2 cl, 3 ex, 3 tbl, 2 dwg
| Title |
Year |
Author |
Number |
| CHIMERA PLASMID PZZSA FOR EXPRESSION OF CHIMERA PROTEIN ANGIOGEN AND STRAIN OF ESCHERICHIA COLI BL21(DE3) PZZSA AS PRODUCER OF RECOMBINANT CHIMERA HUMAN PROTEIN ANGIOGEN |
2002 |
- Mertvetsov N.P.
- Ramazanov Ju.A.
- Majstrenko V.F.
|
RU2221043C2 |
| RECOMBINANT PLASMID DNA PSA V27 ENCODING SYNTHESIS OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII AND BACTERIAL STRAIN ESCHERICHIA COLI AS PRODUCER OF SOLUBLE STREPTAVIDINE FROM STREPTOMYCES AVIDINII |
1999 |
- Gul'Ko L.B.
- Okorokova N.A.
- D'Jakov N.A.
- Sokolov A.K.
- Vejko V.P.
- Debabov V.G.
|
RU2153535C1 |
| RECOMBINANT PLASMID DNA CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE STAPHYLOCOCCUS AUREUS WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH TRIPLETS CODING Ala, STRAINS OF ESCHERICHIA COLI MZ09 AND METHOD OF OBTAINING RECOMBINANT PROTEIN, CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE GENE WITH REPLACEMENT OF CODONS K74, E75 AND R77 WITH Ala-CODING TRIPLETS |
2010 |
- Vil'Kin Jakov Fimovich
- Markin Sergej Sergeevich
- Semenov Andrej Mikhajlovich
|
RU2422153C1 |
| RECOMBINANT PLASMID DNA CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE Staphylococcus aureus WITH IDENTIFICATION PEPTIDE OF SIX HISTIDINE RESIDUES, Escherichia coli MZ08 STRAIN AND METHOD OF PRODUCING RECOMBINANT PROTEIN CONTAINING SEQUENCE OF MATURE STAPHYLOKINASE GENE WITH IDENTIFICATION PEPTIDE OF SIX HISTIDINE RESIDUES |
2009 |
- Vil'Kin Jakov Fimovich
- Markin Sergej Sergeevich
- Semenov Andrej Mikhajlovich
|
RU2422522C2 |
| RECOMBINANT PLASMID pPA-OPRFI DNA CODING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE, STRAIN Escherichia coli PA-OPRFI PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE AND METHOD FOR PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE |
2011 |
- Kaloshin Aleksej Alekseevich
- Mikhajlova Natal'Ja Aleksandrovna
|
RU2537006C2 |
| RECOMBINANT PLASMID DNA PSS5 ENCODING SYNTHESIS OF RECOMBINANT HUMAN ALPHA-2B INTERFERON, STRAIN OF ESCHERICHIA COLI SS5 AS PRODUCER OF RECOMBINANT HUMAN ALPHA-2B INTERFERON AND METHOD OF ALPHA-2B INTERFERON PREPARING |
1999 |
- Cherepanov P.A.
- Mikhajlova T.G.
- Cherepanov P.P.
- Martinenko Dmitrij Leonidovich
- Shevchuk Aleksandr Anatol'Evich
- Fedjukin V.S.
- Nikolaev T.M.
- Tolkachev B.B.
- Sventitskij E.N.
- Urakov N.N.
- Kalinin Ju.T.
- Denisov L.A.
- Tjagotin Ju.V.
- Martjushin S.V.
- Ishchenko A.M.
- Trofimov A.V.
- Poljakova E.A.
- Batarin V.I.
- Shalaeva O.N.
- Lisitskaja V.I.
|
RU2165455C1 |
| METHOD FOR PRODUCING OF HUMAN METHIONINE-FREE INTERFERON-ALPHA2b |
2009 |
- Shirokov Dmitrij Alekseevich
- Rjabichenko Viktor Vasil'Evich
- Akishina Raisa Illarionovna
- Glazunov Aleksandr Viktorovich
- Chestukhina Galina Georgievna
- Vejko Vladimir Petrovich
|
RU2432401C2 |
| METHOD OF PRODUCING OF RECOMBINANT PROTEIN SAV-RGD |
2014 |
- Rubtsov Mikhail Aleksandrovich
- Syrkina Marina Sergeevna
- Vejko Vladimir Petrovich
- Okorokova Natalja Anatolevna
- Zamjatnin Andrej Aleksandrovich
|
RU2577138C1 |
| ARTIFICIAL GENE, CODING CIMERIC PROTEIN OF HUMAN ANGIOGENIN, CHIMERIC PLASMID pJZZ-A, ENSURING EXPRESSION OF GENE OF CHIMERIC PROTEIN OF HUMAN ANGIOGENIN IN Escherichia coli AND STRAIN OF Escherichia coli BL21(DE3)/pJZZ-A-PRODUCENT OF RECOMBINANT CHIMERIC PROTEIN OF HUMAN ANGIOGENIN |
2012 |
- Ramazanov Jurij Akhmetovich
- Beklemishev Anatolij Borisovich
|
RU2512527C2 |
| RECOMBINANT PLASMIDE DNA PPR-TGATG-HIL-Iβ-TSR WHICH PROVIDES SYNTHESIS OF RECOMBINANT INTERLEIKIN-1 AND STRAIN BSCHERICHIA COLI BEING PRODUCER OF RECOMBINANT HUMAN INTERLEIKIN-1 b |
1992 |
- Mashko S.V.
- Lebedeva M.I.
- Vinetskij Ju.P.
- Kotenko S.V.
- Ketlinskij S.A.
- Simbirtsev A.S.
- Kozlov A.P.
- Izotova L.S.
- Kozlov Ju.I.
- Debabov V.G.
|
RU2045575C1 |
