FIELD: biotechnology, microbiology.
SUBSTANCE: method involves using an encapsulated adsorbent and/or antioxidant in preparing seeding material and submerged culturing. In preparing seeding material the culturing process is carried out in freshly prepared nutrient medium up to the stationary growth phase. Submerged culturing is carried out by simultaneous inoculation of bioreactor with seeding material and contacting the content of bioreactor with encapsulated adsorbent and/or antioxidant. The oxygen content in bioreactor is maintained at the level 5-10% of the equilibrium level. Invention provides increasing yield of halophilic microorganisms biomass up to 42 g/l and with the content of bacteriorhodopsin up to 1.6 g/l and in practically absent of carotinoids in biomass. Invention provides reducing time for preparing the unit mass of the end product and to decrease consumptions for production of bacteriorhodopsin.
EFFECT: improved preparing method of biomass.
1 tbl, 10 ex
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Authors
Dates
2008-04-27—Published
2006-05-30—Filed