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2 743 861

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IPC

C12N15/11(2006-01-01)

C12N15/113(2010-01-01)

C12N9/22(2006-01-01)

C12Q1/6816(2018-01-01)

C12Q1/6844(2018-01-01)

C12Q1/6876(2018-01-01)

C12Q1/6888(2018-01-01)

(21) (22)

Application

2020114570, 2020-04-15

(24)

Start date

2020-04-15

(22)

Actual filing date

2020-04-15

(45)

Published

2021-03-01

(72)

Inventor

Akimkin Vasilij GennadevichTyumentsev Aleksandr IgorevichTyumentseva Marina Alekseevna

(73)

Holder

Federalnoe Byudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatelskij Institut Epidemiologii" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka Tsnii Epidemiologii Rospotrebnadzora)

CRISPR-CAS SYSTEM FOR DETECTING THE ANTIBIOTIC RESISTANCE GENE BLAVIM-2 (METALLO-BETA-LACTAMASE CLASS B VIM-2) PSEUDOMONAS AERUGINOSA AT ULTRA-LOW CONCENTRATIONS Russian patent published in 2021 - IPC C12N15/11 C12N15/113 C12N9/22 C12Q1/6816 C12Q1/6844 C12Q1/6876 C12Q1/6888

Abstract RU 2743861 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely to a molecule of the guide RNA system CRISPR/CAS. Also disclosed a ribonucleoprotein complex of the CRISPR/CAS system for detecting the antibiotic resistance gene blaVIM-2 of Pseudomonas aeruginosa, formed from the RNA directed DNA endonuclease LbCpf1 from Lachnospiraceae and at least one of the above targeting RNAs. The invention also relates to a kit containing the above molecule or the above complex.

EFFECT: provides detection of single copies of the antibiotic resistance gene blaVIM-2 Pseudomonas aeruginosa.

7 cl, 13 dwg, 5 tbl, 5 ex

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RU 2 743 861 C1

Authors

Akimkin Vasilij Gennadevich

Tyumentsev Aleksandr Igorevich

Tyumentseva Marina Alekseevna

Dates

2021-03-01—Published

2020-04-15—Filed