FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, namely to a molecule of the guide RNA system CRISPR/CAS. Also disclosed a ribonucleoprotein complex of the CRISPR/CAS system for detecting the antibiotic resistance gene blaVIM-2 of Pseudomonas aeruginosa, formed from the RNA directed DNA endonuclease LbCpf1 from Lachnospiraceae and at least one of the above targeting RNAs. The invention also relates to a kit containing the above molecule or the above complex.
EFFECT: provides detection of single copies of the antibiotic resistance gene blaVIM-2 Pseudomonas aeruginosa.
7 cl, 13 dwg, 5 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING THE ANTIBIOTIC RESISTANCE GENE BLAVIM-2 (METAL-BETA-LACTAMASE CLASS B VIM-2) PSEUDOMONAS AERUGINOSA IN ULTRALOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2020 |
|
RU2734520C1 |
| METHOD FOR OBTAINING THE CRISPR/CAS RIBONUCLEOPROTEIN COMPLEX PREPARATION AND PREPARATION FOR DETECTION OF THE BLAVIM-2 (METAL-BETA-LACTAMASE CLASS B VIM-2) PSEUDOMONAS AERUGINOSA ANTIBIOTIC RESISTANCE GENE IN ULTRA-LOW CONCENTRATIONS | 2020 |
|
RU2745637C1 |
| METHOD FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN METHOD | 2023 |
|
RU2820306C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839482C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA, AT ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2791879C1 |
| METHOD FOR PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND A PREPARATION FOR DETECTING THE exoU GENE ENCODING AN EXOTOXIN OF THE THIRD TYPE SECRETION SYSTEM, PSEUDOMONAS AERUGINOSA | 2021 |
|
RU2782739C1 |
| METHOD FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA AT ULTRALOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2791880C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839762C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-OXA-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841370C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2829103C1 |
