FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to a CRISPR-Cas system guide RNA molecule. Said molecule is capable of binding to target highly conserved regions of the antibiotic resistance gene bla-TEM-IB, where the nucleotide sequence of the guide RNA is selected from sequences SEQ ID NO: 1-6. Invention also relates to a ribonucleoprotein complex of the CRISPR-Cas system, as well as kits containing such a complex, which are intended for detecting the antibiotic resistance gene bla-TEM-1B.
EFFECT: invention provides higher efficiency of detecting antibiotic resistance gene bla-TEM-1B up to 1,5 times.
7 cl, 10 dwg, 4 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
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|
RU2820307C1 |
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| CRISPR-CAS12 SYSTEM FOR DETECTING THE MECA ANTIBIOTIC RESISTANCE GENE OF STAPHYLOCOCCUS AUREUS AT ULTRA-LOW CONCENTRATIONS | 2021 |
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RU2782588C1 |
| METHOD OF DETECTION OF RNA OF HEPATITIS C VIRUS GENOTYPES 1B AND 3A IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2022 |
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RU2800420C1 |
| CRISPR-CAS12 SYSTEM FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA, AT ULTRA-LOW CONCENTRATIONS | 2021 |
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| METHOD FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA AT ULTRALOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
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RU2791880C1 |
