FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to a CRISPR-Cas system guide RNA molecule. Said molecule is capable of binding to target highly conserved regions of the antibiotic resistance gene bla-TEM-IB, where the nucleotide sequence of the guide RNA is selected from sequences SEQ ID NO: 1-6. Invention also relates to a ribonucleoprotein complex of the CRISPR-Cas system, as well as kits containing such a complex, which are intended for detecting the antibiotic resistance gene bla-TEM-1B.
EFFECT: invention provides higher efficiency of detecting antibiotic resistance gene bla-TEM-1B up to 1,5 times.
7 cl, 10 dwg, 4 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2820307C1 |
| METHOD FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN METHOD | 2023 |
|
RU2820306C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839484C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839761C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-NDM-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841369C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839482C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839763C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839762C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839486C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-OXA-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841370C1 |
