FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a molecule of RNA guide of CRISPR-Cas system. Said molecule is capable of binding to target highly conserved regions of the bla-OXA-1 antibiotic resistance gene and is characterized by a nucleotide sequence selected from SEQ ID NO: 1-3. In addition, invention also relates to ribonucleoprotein complex of CRISPR-Cas system for detecting antibiotic resistance gene bla-OXA-1, formed from RNA-guided DNA endonuclease LbCpf1 from Lachnospiraceae and at least one of said RNA guides.
EFFECT: present invention provides a considerably more effective detection of the antibiotic resistance gene bla-OXA-1.
5 cl, 7 dwg, 4 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839763C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839486C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-OXA-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841370C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839484C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839761C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-NDM-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841369C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2829103C1 |
| METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2820307C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839482C1 |
| METHOD FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN METHOD | 2023 |
|
RU2820306C1 |
