FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular, to a guide RNA molecule of the CRISPR-Cas system, a ribonucleoprotein complex of the CRISPR-Cas system containing the guide RNA molecule, and to kits containing a ribonucleoprotein complex of the CRISPR-Cas system and specific oligonucleotides.
EFFECT: invention provides the in vitro detection of single copies of the exoU gene of Pseudomonas aeruginosa.
10 cl, 9 dwg, 6 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING THE EXOU GENE ENCODING THE TYPE 3 SECRETION SYSTEM EXOTOXIN, PSEUDOMONAS AERUGINOSA AT ULTRALOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2791880C1 |
| METHOD FOR PRODUCING A PREPARATION OF A RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND A PREPARATION FOR DETECTING THE exoU GENE ENCODING AN EXOTOXIN OF THE THIRD TYPE SECRETION SYSTEM, PSEUDOMONAS AERUGINOSA | 2021 |
|
RU2782739C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839761C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTION OF ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839762C1 |
| CRISPR-Cas12 SYSTEM FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS | 2023 |
|
RU2829103C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839484C1 |
| METHOD FOR PRODUCING PREPARATION OF RIBONUCLEOPROTEIN CRISPR-Cas COMPLEX AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS | 2024 |
|
RU2839763C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-OXA-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839486C1 |
| METHOD OF DETECTING THE ANTIBIOTIC RESISTANCE GENE BLA-NDM-1 IN ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2024 |
|
RU2841369C1 |
| CRISPR-Cas12 KIT FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-NDM-1 IN ULTRA-LOW CONCENTRATIONS (VERSIONS) | 2024 |
|
RU2839482C1 |
