FIELD: biotechnology.
SUBSTANCE: proposed is a pUSB2-AmQ plasmid. The size of the pUSB2-AmQ plasmid is 7495 base pairs. The pUSB2-AmQ plasmid is comprised of a number of structural elements. A synthetic promoter provides efficient transcription of controlled mRNA. The recombinant B. amyloliquefaciens α-amylase gene encodes a target recombinant protein. The bla sequence encodes a beta-lactamase protein. The bla sequence is a selectable marker for transformation of E. coli strains. The repB section provides replication initiation in B. subtilis strains. Chloramphenicolacetylase is a selectable marker for transformation of B. subtilis strains. The recombinant Bacillus subtilis strain WB800N/pUSB2-AmQ is obtained through transformation of the original strain by the pUSB2-AmQ plasmid. The strain provides production of α-amylase.
EFFECT: during cultivation, the Bacillus subtilis WB800N/pUSB2-AmQ strain provides synthesis of recombinant alpha-amylase of 90% of the total secreted Bacillus subtilis protein.
2 cl, 7 dwg, 5 tbl, 4 ex
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