FIELD: biotechnology, biochemistry, enzymology.
SUBSTANCE: variant of the parent Termamil-like alpha-amylase comprises change in one or some positions of the parent alpha-amylase. Such changes represent insertions, deletions and substitutions of amino acids in amino acid sequence of this enzyme. Indicated variant retains activity of alpha-amylase and elicits alternated properties such as reduced capacity for substrate cleavage near the branching point and improved substrate specificity. Invention enhances effectiveness in using alpha-amylase in detergent compositions, in manufacturing ethanol, for treatment of fabrics or dressings.
EFFECT: valuable properties of enzyme.
13 cl, 5 ex
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Authors
Dates
2004-06-27—Published
2000-03-28—Filed