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2 833 838

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C1

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IPC

C12N1/19(2006-01-01)

C12N9/28(2006-01-01)

C12N15/56(2006-01-01)

C12N15/81(2006-01-01)

(21) (22)

Application

2023131758, 2023-11-29

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Start date

2023-11-29

(22)

Actual filing date

2023-11-29

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Published

2025-01-29

(72)

Inventor

Kulminskaya Anna AlekseevnaKorolev Vladimir GennadevichPeshekhonov Vyacheslav TimofeevichEnejskaya Elena VladimirovnaEvstyukhina Tatyana AnatolevnaBobrov Kirill SergeevichKorban Svetlana Andreevna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Institut Yadernoj Fiziki Im. B.P. Konstantinova Natsionalnogo Issledovatelskogo Tsentra Institut" Institut" Piyaf)

RECOMBINANT PLASMID FOR EXPRESSION OF ALPHA-AMYLASE FROM BACILLUS SPECIES H3 AND SACCHAROMYCES CEREVISIAE YEAST STRAIN - PRODUCER OF ALPHA-AMYLASE FROM BACILLUS SPECIES H3, HAVING CONTROLLED MUTABILITY Russian patent published in 2025 - IPC C12N1/19 C12N9/28 C12N15/56 C12N15/81 

Abstract RU 2833838 C1

FIELD: biotechnology.

SUBSTANCE: disclosed is a recombinant plasmid for expressing alpha-amylase from Bacillus sp. H3, carrying a gene coding alpha-amylase from Bacillus sp. H3, having size of 7945 base pairs, obtained on the basis of pYES2 and characterized by a physical and genetic map presented in Fig. 1. Also disclosed is a Saccharomyces cerevisiae yeast strain – an alpha-amylase producer from Bacillus sp. H3, having controlled mutability, obtained by transforming a Saccharomyces cerevisiae strain having a genotype MATα ade2-248 leu2-3,112 ura3-160.188 radlΔ hmolΔ, said recombinant plasmid.

EFFECT: invention enables to obtain an alpha-amylase producer strain from Bacillus species H3 based on Saccharomyces cerevisiae yeast with controlled mutability.

2 cl, 2 dwg, 5 tbl

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RU 2 833 838 C1

Authors

Kulminskaya Anna Alekseevna

Korolev Vladimir Gennadevich

Peshekhonov Vyacheslav Timofeevich

Enejskaya Elena Vladimirovna

Evstyukhina Tatyana Anatolevna

Bobrov Kirill Sergeevich

Korban Svetlana Andreevna

Dates

2025-01-29—Published

2023-11-29—Filed

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