FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology. A recombinant Ogataea haglerorum strain VKPM Y-4639 is proposed, which is an-mannanase producer and contains an optimized sequence of the gene encoding Bacillus subtilis β-mannanase EC 3.2.1.78 in the chromosome. When the strain is cultivated in flasks, about 1.0–0.2 mg / ml accumulates in the culture liquid, the activity of the β-mannanase enzyme is 12000–500 U / ml. When cultivated in a fermenter, the inventive strain secretes a protein of 4.25 g / l, and the activity of β-mannanase is 51066 units / ml for 160 hours.
EFFECT: invention provides an expansion of the arsenal of recombinant microorganisms producing β-mannanase.
1 cl, 3 tbl, 4 ex, 1 dwg
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