FIELD: biotechnology.
SUBSTANCE: Komagataella phaffii yeast transformant producing an active form of Vicugna pacos alpaca chymosin and containing in chromosomal DNA an optimized synthetic Vicugna pacos alpaca prochymosin gene, the nucleotide sequence of which is given in the sequence listing under the following SEQ ID NO: 1. Also the strain Ogataea haglerorum N414 “ВКМП” Y-5077, which produces recombinant chymosin from alpaca Vicugna pacos in an active form is proposed.
EFFECT: expansion of the range of yeast recombinant microorganisms that produce Vicugna pacos alpaca chymosin in active form.
2 cl, 6 dwg, 4 tbl, 6 ex
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