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2 748 979

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(51)

IPC

C12Q1/68(2006-01-01)

G01N33/48(2006-01-01)

C12Q1/686(2018-01-01)

A61B1/267(2006-01-01)

A61B8/00(2006-01-01)

(21) (22)

Application

2020110166, 2020-03-10

(24)

Start date

2020-03-10

(22)

Actual filing date

2020-03-10

(45)

Published

2021-06-02

(72)

Inventor

Shabunin Sergej ViktorovichParshin Pavel AndreevichVostroilova Galina AnatolevnaSavrasov Dmitrij AleksandrovichSyromyatnikov Mikhail YurevichMikhajlov Evgenij VladimirovichPasko Nadezhda ValerievnaStrebkova Vera ValentinovnaPonomarev Artem Olegovich

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Nauchnoe Uchrezhdenie Nauchno-Issledovatelskij Veterinarnyj Institut Patologii, Farmakologii I Terapii"

METHOD FOR DIAGNOSIS OF CALF MALNUTRITION (BOS TAURUS) BASED ON ANALYSING EXPRESSION OF PRO-INFLAMMATORY CYTOKINE GENE OF INTERLEUKIN IL1Α Russian patent published in 2021 - IPC C12Q1/68 G01N33/48 C12Q1/686 A61B1/267 A61B8/00

Abstract RU 2748979 C1

FIELD: biotechnology; veterinary science.

SUBSTANCE: present invention relates to biotechnology and veterinary science, can be used for diagnosing calf hypotrophy. Method comprises extracting total RNA from collected calf blood, then cDNA is obtained. That is followed by PCR with cDNA, interleukin 1α gene expression is calculated in relation to housekeeping gene expression by indicator 2^-ΔΔct, where ΔΔCt = ΔCt (calf with suspected hypotrophy) - ΔCt (healthy calf); ΔCt = Ct (interleukin 1α gene) - Ct (β-actin gene). Intercalating colouring agent used in PCR is SYBR Green.

EFFECT: invention enables assessing the risk of developing hypotrophy in calves within 4–5 hours after blood sampling.

1 cl, 3 dwg, 1 tbl, 1 ex

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RU 2 748 979 C1

Authors

Shabunin Sergej Viktorovich

Parshin Pavel Andreevich

Vostroilova Galina Anatolevna

Savrasov Dmitrij Aleksandrovich

Syromyatnikov Mikhail Yurevich

Mikhajlov Evgenij Vladimirovich

Pasko Nadezhda Valerievna

Strebkova Vera Valentinovna

Ponomarev Artem Olegovich

Dates

2021-06-02—Published

2020-03-10—Filed