FIELD: biotechnology.
SUBSTANCE: invention relates to recombinant plasmid DNA encoding human antithrombin III minigene under the transcriptional control of regulatory elements of the mouse Serpinc1 gene, the polyadenylation signal of the SV40 virus and the transcription terminator from the human SKIV2L gene, flanked by homology arms of the mouse Serpinc1 locus with a mutated PAM site. Also the following is disclosed: a method of producing transgenic mice expressing the human antithrombin III minigene involving the use of the said plasmid DNA.
EFFECT: invention is effective for producing genetically modified mice humanized by Serpinс1 gene.
2 cl, 6 dwg, 2 tbl, 8 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR CONSTRUCTING MAMMALIAN MINIGENES AND RECOMBINANT PLASMID PGC1HDR ENCODING THE HUMAN C1 ESTERASE INHIBITOR MINIGENE INTENDED FOR PRODUCING MICE HUMANIZED BY THE SERPING1 GENE | 2022 |
|
RU2805177C1 |
| METHOD OF OBTAINING GENETICALLY MODIFIED LABORATORY ANIMALS WITH INDUCIBLE SYSTEMIC AND TISSUE-SPECIFIC EXPRESSION OF HUMAN CYCLOPHILIN A | 2022 |
|
RU2800264C1 |
| METHOD OF PRODUCING MOUSE MODEL FOR STUDYING DUCHENNE MUSCULAR DYSTROPHY AND VERSIONS OF ITS THERAPY | 2023 |
|
RU2815936C1 |
| CRISPR-CAS SYSTEMS AND METHODS OF CHANGING EXPRESSION OF GENE PRODUCTS | 2013 |
|
RU2687451C1 |
| TECHNOLOGY FOR OBTAINING MICE WITH A HUMANIZED GNAO1 REGION IN THE REGION OF SINGLE NUCLEOTIDE POLYMORPHISM rs587777057 FOR TESTING RNA THERAPY FOR GNAO1 c.607 G>A PATIENTS | 2021 |
|
RU2757121C1 |
| METHOD FOR OBTAINING A MOUSE MODEL FOR STUDYING LESCH-NYHAN SYNDROME BY INTRODUCING A DELETION OF P.Val8del INTO THE Hprtl GENE | 2021 |
|
RU2768048C1 |
| METHOD FOR PRODUCING GENETICALLY MODIFIED LABORATORY ANIMALS WITH NULL ALLELE OF P2rx3 GENE | 2022 |
|
RU2805173C1 |
| GENETIC CONSTRUCTS LTF3, LTF5, LTF7, LTF10, LTF11 FOR PRODUCING RECOMBINANT HUMAN LACTOFERRIN (VERSIONS) | 2011 |
|
RU2491343C1 |
| METHOD OF PRODUCING DIMERIC FORM OF IMMUNOGLOBULIN IGA1-ISOTYPE IN MAMMALIAN CELLS | 2023 |
|
RU2822890C1 |
| TRANSGENE FOR PREPARING THE RECOMBINANT POLYPEPTIDE IN TRANSGENIC COW MILK, A METHOD OF OBTAINING THE TRANSGENIC COW (VARIANTS), MILK FROM TRANSGENIC COW, FOOD COMPOSITION | 1990 |
|
RU2095414C1 |
