HYBRID CARRIERS FOR DELIVERY OF NUCLEIC ACIDS Russian patent published in 2021 - IPC A61K47/64 A61K48/00 A61P1/00 A61P3/00 A61P11/00 A61P17/00 A61P21/00 A61P25/00 A61P27/00 A61P31/00 A61P33/00 A61P35/00 A61P37/00 A61P43/00 C07C215/14 C07C233/20 C07C233/56 C07C237/10 

FIELD: medicine.

SUBSTANCE: invention relates to a composition for the delivery of nucleic acids. The composition contains (a) a cationic peptide or polymer; (b) a cationic or permanently cationic lipidoid compound; and (c) a nucleic acid compound. In this case, the specified cationic or permanently cationic lipidoid compound contains a central nitrogen atom connected with С_2-3 alkylene groups with three groups of Formula IIa and/or Formula IIb: -N(R₁)-CH₂-CH(R₅)-R₂ (Formula IIa), -N⁺(R₃)(R₄)-CH₂-CH(R₅)-R₂ (Formula IIb), and independently for each individual group of Formula IIa or Formula IIb R₁ is selected from hydrogen or C₁-C₄-alkyl, R₂ is selected from linear C₆, С₈ or С₁₀-alkyl or branched, saturated, or unsaturated C₆-C₁₆ hydrocarbyl chain, R₃ and R₄ are selected from C₁-C₄-alkyl and R₅ is a hydrogen or hydroxyl. The specified cationic peptide or polymer is a compound containing at least one cationic group P, containing at least one group-SH, capable of forming a disulfide bond, or its disulfide-coupled multimer. Group P is selected from a polymer group having a molecular weight of 0.5 kDa to 30 kDa, or a peptide group consisting of 3-100 amino acids, with at least 10% of the total amino acids of the specified peptide group being the main amino acids selected from Arg, Lys, His and/or Orn. The invention also relates to nanoparticles for the delivery of nucleic acids containing the specified composition, a set for the preparation of the composition, a vaccine and a method of delivery and RNA to the eye.

EFFECT: invention provides high efficiency of the composition, and ability to deliver nucleic acids to mammalian cells.

34 cl, 11 dwg, 8 tbl, 11 ex