FIELD: biotechnology.
SUBSTANCE: group of inventions relates to the field of biological detection. Disclosed is a cartridge for semiquantitative detection of human chorionic gonadotropin, containing at least a first strip of indicator paper (11), a second strip of indicator paper (12), a third strip of indicator paper (13), a fourth strip of indicator paper (14), a fifth strip of indicator paper (15) and a sixth strip of indicator paper (16), wherein each of the strips includes a substrate (1) and a sample pad (2), an adsorbent pad for colloidal gold (3), an antibody-carrying film (4) and an adsorbent pad for water (5), glued sequentially to the substrate (1); the antibody-carrying film (4) has a detection line (6) at the end close to the adsorbent pad for colloidal gold (3) and a quality control line (7) at the end close to the adsorbent pad for water (5); the detection line (6) is coated with a monoclonal antibody to human chorionic gonadotropin α-HCG; the quality control line (7) is coated with a polyclonal antimouse IgG antibody; the adsorbent pad for colloidal gold (3) of the first strip of indicator paper (11) has an adsorbed conjugate of a monoclonal antibody to human chorionic gonadotropin β-HCG with colloidal gold, and the adsorbent pads for colloidal gold (3) of the second strip of indicator paper (12), the third strip indicator paper (13), the fourth strip of indicator paper (14), the fifth strip of indicator paper (15) and the sixth strip of indicator paper (16) have an adsorbed conjugate of a monoclonal antibody to human chorionic gonadotropin β-HCG with colloidal gold and a free β-antibody bound with the HCG from the sample, with a mass ratio of 1:1, 1:2, 1:40, 1:80, 1:100, respectively; wherein the free β-antibody is a non-mouse antibody. Also disclosed are a method for manufacturing a cartridge, a reagent plate and an application of said cartridge or plate for determining the range of concentrations of human chorionic gonadotropin in a urine sample.
EFFECT: group of inventions provides a wide range of determined concentrations, high accuracy and sensitivity of analysis.
15 cl, 7 dwg, 1 tbl, 7 ex
