FIELD: medical biotechnology.
SUBSTANCE: invention relates to medical biotechnology. The invention is a method for detecting hepatitis B virus DNA in a biological material based on a two-stage polymerase chain reaction (PCR) with real-time detection. Detection is carried out in plasma and / or serum, blood clot, liver tissue. Diagnostics is carried out in two stages. At the first stage, amplification of the virus DNA is carried out using oligonucleotide primers complementary to the regions of greatest similarity of genomes of different HBV isolates in the regions of the S gene and X gene (forward primer 
reverse primer 
At the second stage, two pairs of oligonucleotides are used to two regions (gene S and gene X) of the virus genome and the corresponding oligonucleotide fluorescently labeled probes, complementary to the regions of the amplified fragments, carrying fluorophores at the 5 'end, and not fluorescent quenchers at the 3' end. The results obtained are recorded by means of real-time hybridization-fluorescence detection.
EFFECT: method improves sensitivity and reliability of diagnostics.
1 cl, 1 dwg, 2 tbl, 4 ex
