FIELD: biotechnology.
SUBSTANCE: described is a method for detecting Mammarenavirus guanaritoense virus using the DETECTR method, involving extraction of RNA from biological samples followed by detection of target RNA fragments of the viral genome. Method comprises performing isothermal recombinase polymerase amplification with reverse transcription, combined in one test tube with fluorescent detection, by preliminary spatial separation of reaction mixtures for amplification of nucleic acids and fluorescent detection DETECTR in a test tube, followed by their mixing, using LbaCas12a nuclease, primers and a probe.
EFFECT: wider range of products used for diagnosing, monitoring and preventing possible future epidemics, providing reliable diagnosis of the presence of Mammarenavirus guanaritoense virus RNA.
1 cl, 1 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR DETECTING MAMMARENAVIRUS MACHUPOENSE BY DETECTR METHOD WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2832917C1 |
| METHOD FOR DETECTING HENIPAVIRUS HENDRAENSE BY DETECTR METHOD WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2834907C1 |
| METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | 2024 |
|
RU2834909C1 |
| METHOD FOR DETECTING RNA OF HENIPAVIRUS HENDRAENSE VIRUS BY RECOMBINASE POLYMERASE AMPLIFICATION | 2024 |
|
RU2834950C1 |
| METHOD OF DETECTING GUANARITO VIRUS BY METHOD BASED ON USE OF DEOXYRIBOZYME 10-23 | 2024 |
|
RU2827922C1 |
| METHOD FOR DETECTING JUNIN VIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822164C1 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR | 2024 |
|
RU2834908C1 |
