FIELD: biotechnology.
SUBSTANCE: described is a method for detecting Junin virus RNA by real-time RT-PCR, involving extraction of RNA from biological samples, followed by a two-step RT-PCR reaction for the target RNA fragments of the viral genome using a set of oligonucleotide primers and a corresponding fluorescent-labelled probe, complementary to a portion of the L gene coding the RNA-dependent RNA polymerase of the Junin virus, sequence is used as a forward primer tgggctgataaacttgtttaatttag, as a reverse primer – tgatgtgatacctgtcacttatac, and as a probe – a sequence tcatggaagcacaccatttccagcag, in which a fluorescent label R6G is located at 5'-end, and a non-fluorescent quencher BHQ1 is located at 3'-end.
EFFECT: method is intended for monitoring and diagnosing Junin virus in healthy people, patients and individuals with suspected haemorrhagic fever caused by Junin virus.
1 cl, 1 dwg, 3 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETECTING RNA OF VIRUS OF CRIMEAN-CONGO HAEMORRHAGIC FEVER USING REAL-TIME RT-PCR | 2024 |
|
RU2834908C1 |
| METHOD FOR DETECTING HENDRA HENIPAVIRUS RNA BY REAL-TIME RT-PCR | 2023 |
|
RU2822161C1 |
| METHOD FOR DETECTING BANDAVIRUS DABIEENSE (SFTSV) VIRUS RNA BY REAL-TIME RT-PCR | 2024 |
|
RU2831410C1 |
| METHOD FOR DETECTING RNA OF MAMMARENAVIRUS GUANARITOENSE VIRUS BY REAL-TIME RT-PCR | 2024 |
|
RU2839982C1 |
| METHOD FOR DETECTING MODIFIED POLIOVIRUS VACCINE TYPE 2 (nOPV2) RNA BY REAL-TIME RT-PCR | 2022 |
|
RU2795703C1 |
| METHOD OF DETECTING NIPAH VIRUS USING REAL-TIME RT-PCR | 2022 |
|
RU2816270C2 |
| METHOD FOR DETECTING MEASLES VIRUS BY REAL-TIME RT-PCR | 2023 |
|
RU2822430C1 |
| METHOD OF DETECTING MONKEY POX VIRUS OF MONKEYPOX TYPE BY REAL TIME PCR (MPX AMP PS) | 2023 |
|
RU2803898C1 |
| SET FOR DETECTING SARS-COV VIRUS BY REAL-TIME RT-PCR METHOD | 2020 |
|
RU2744198C1 |
| METHOD FOR DETECTING MAMMARENAVIRUS MACHUPOENSE BY DETECTR METHOD WITH ISOTHERMAL AMPLIFICATION | 2024 |
|
RU2832917C1 |
