FIELD: medicine.
SUBSTANCE: method for hepatitis B virus identification at a low viral load is proposed by virus DNA determination in a bioassay using the PCR method with further fragments sequencing. Detection is carried out in plasma and/or serum, blood clots, oropharyngeal washings, sperm, liver tissues. Identification is carried out in two stages. At the first stage, asymmetric PCR with extended primers is used (direct primer: 5'-ctgcgcaccagcaccatgcaactttttcacctctgc-3', reverse primer: 5'-cagaccaatttatgcctacccccca-3'). The second stage uses primers to one of the four regions of the HBV genome.
EFFECT: increased specificity and sensitivity of hepatitis B virus diagnosis.
4 ex, 2 dwg, 2 tbl
