FIELD: medical genetics.
SUBSTANCE: invention relates to the field of medical genetics, molecular biology and can be used in oncology and hematology for the rapid detection of recurrent mutation p.L265P in the MYD88 gene using standard equipment in a wide clinical practice. DNA is isolated from the sample taken from the patient. The direct (5'-GTACTTAGATGGGGGATGGCTGTTG-3') and reverse (5'-ATTGCCTTGTACTTGATGGG ATG-3') primers flanking the region of the p.L265P mutation are used to perform PCR amplification of the MYD88 gene region potentially containing the p.L265P mutation. The resulting amplicon is treated with HinfI restriction endonuclease. Electrophoresis of restriction products is performed and when alleles with a length of 148 bp are detected; 148 bp, 124 bp and 24 bp; 124 bp and 24 bp, respectively, the p.L265P mutation in the homozygous, heterozygous state and its absence are diagnosed. The method is applicable for freshly collected, frozen biomaterial, FFPE samples, including archival ones, most of whose DNA fragments are about 200 bp long.
EFFECT: method is more sensitive compared to the known method for PCR-RFLP analysis of FFPE samples; it is simple, performed within 2 days on equipment available in clinical practice.
1 cl, 2 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD FOR IDENTIFYING THE Cys1079Gly AND Cys1079Phe POLYMORPHISMS OF WILSON COPPER-TRANSPORTING ATPase | 2020 |
|
RU2756112C1 |
METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN BRAF, NRAS AND KIT GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
|
RU2674338C1 |
METHOD FOR IDENTIFICATION OF BRAF GENE MUTATIONS IN HUMAN TUMOURS | 2016 |
|
RU2631824C1 |
METHOD FOR ANALYSIS OF SOMATIC MUTATIONS IN GNAQ AND GNA11 GENES USING LNA-BLOCKING MULTIPLEX PCR AND SUBSEQUENT HYBRIDIZATION WITH OLIGONUCLEOTIDE BIOLOGICAL MICROCHIP (BIOCHIP) | 2017 |
|
RU2674687C1 |
METHOD OF IDENTIFICATION OF MUTATIONS 2282del4, R501X, R2447X IN GENES OF FILAGGRIN (FLG) WITH VULGAR ICHTYOSIS AND ATOPIC DERMATITIS | 2018 |
|
RU2673804C1 |
METHOD FOR CARRYING OUT PCR WITH ALLELE-SPECIFIC PROBES FOR GENOTYPING CATTLE BY THE ALLELES A AND K OF THE DGAT1 GENE | 2018 |
|
RU2662972C1 |
METHOD FOR ANALYSING SOMATIC MUTATIONS IN IDH1 AND IDH2 GENES USING LNA-BLOCKING PCR AND HYBRIDISATION WITH BIOLOGICAL MICROCHIP | 2024 |
|
RU2839291C1 |
SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR GENETIC TYPING OF POLYMORPHOUS DNA LOCI ASSOCIATED WITH A RISK OF PROGRESSION OF SPORADIC FORM OF ALZHEIMER'S DISEASE IN RUSSIAN POPULATIONS | 2014 |
|
RU2600874C2 |
METHOD FOR PREDICTION OF RISK OF RENAL CELL CARCINOMA | 2019 |
|
RU2721709C1 |
METHOD FOR C-KIT GENE L576P MUTATION DIAGNOSTIC IN MELANOMA TISSUE | 2016 |
|
RU2627440C2 |
Authors
Dates
2021-10-06—Published
2020-11-25—Filed