FIELD: biotechnology.
SUBSTANCE: invention represents a diagnostic technique for predisposition to renal cell carcinoma based on PCR-RFLP, which consists in DNA extraction from pre-selected biological material of patients with PCR reaction, using primers GCAGGCCTCTCTGTCTGAAC (SEQ ID NO: 2) and GATGACATGTGGGTGGTTGA (SEQ ID NO: 2), as well as the restriction analysis of the obtained amplicon. Restriction enzyme Tsel is used for restriction of the amplicon. Restriction enzyme forms unique restriction fragments in the presence of mutations and normal (31 bp, 61 bp and 104 bp in norm; 92 bp and 104 bp in the presence of a mutation). If observing a mutation during the reaction, a person in the European population has predisposition to renal cell carcinoma.
EFFECT: invention enables detecting predispositions to renal cell carcinoma in a human from the European population for 4½–6 hours (depending on the selected DNA extraction method) without the need to sequencing DNA samples.
1 cl
Title | Year | Author | Number |
---|---|---|---|
DIAGNOSTIC TECHNIQUE FOR PREDISPOSITION TO RENAL CELL CARCINOMA BASED ON PCR-RFLP | 2019 |
|
RU2723090C1 |
DIAGNOSTIC METHOD OF PREDISPOSITION TO BREAST CANCER IN RUSSIAN POPULATION BASED ON PCR-RFLP | 2018 |
|
RU2723585C2 |
METHOD FOR DETECTING p.L265P MUTATION IN MYD88 GENE | 2020 |
|
RU2756909C1 |
METHOD OF DIFFERENTIATED USE OF DNA SEQUENCING DATA FOR PATIENTS AFTER KIDNEY TRANSPLANTATION | 2022 |
|
RU2803796C1 |
METHOD FOR IDENTIFYING THE Cys1079Gly AND Cys1079Phe POLYMORPHISMS OF WILSON COPPER-TRANSPORTING ATPase | 2020 |
|
RU2756112C1 |
DETERMINATION OF CANCER SUSCEPTIBILITY BY IDENTIFICATION OF GENOTYPIC COMBINATIONS OF SPECIFIC VERSIONS OF CYP1B1, BRCA2 AND CHEK2 GENES | 2006 |
|
RU2470998C2 |
METHOD OF GENOTYPING HUMAN PEROXIREDOXINE-1 rs17522918 GENE POLYMORPHISM | 2011 |
|
RU2458145C1 |
BEDBUGS HARMFUL BUG (EURYGASTER INTEGRICEPS PUTON, 1881) IDENTIFICATION METHOD BASED ON GENE RESTRICTION ANALYSIS OF MITOCHONDRIAL DNA CYTOCHROME OXIDASE | 2016 |
|
RU2617935C1 |
METHOD OF GENOTYPING HUMAN THIOREDOXINEREDUCTASE-1 rs1128446 GENE POLYMORPHISM | 2011 |
|
RU2458146C1 |
METHOD OF DIAGNOSING HEREDOTARY PREDISPOSITION TO THROMBOPHILIA | 2007 |
|
RU2352641C1 |
Authors
Dates
2020-05-21—Published
2019-09-25—Filed