METHOD FOR PRODUCING A HIGHLY PURIFIED TETRADECAPEPTIDE Russian patent published in 2021 - IPC C07K7/08 C07K1/10 

FIELD: chemistry.

SUBSTANCE: invention relates to peptide and pharmaceutical chemistry, namely to a method for producing a tetradecapeptide H-Thr-Glu-Lys-Lys-Arg-Arg-Glu-Thr-Val-Glu-Arg-Glu-Lys-Glu-OH by the reaction of condensation of a pentapeptide by the formula Boc-Thr-Glu(OtBut)-Lys(Boc)-Lys(Boc)-Arg-OH with a nonapeptide with a free amino group by the formula H-Arg-Glu(OtBut)-Thr-Val-Glu(OtBut)-Arg-Glu(OtBut)-Lys(Boc)-Glu(OtBut)-OtBut, wherein the pentapeptide Boc-Thr-Glu(OtBut)-Lys(Boc)-Lys(Boc)-Arg-OH is produced from arginine by subsequent addition of N-succinimidyl ether of Nα-carbobenzoxy-Nε-tert-butyloxycarbonyl-L-lysine, N-Succinimidyl ether of Nα-carbobenzoxy-Nε-tert-butyloxycarbonyl-L-lysine, N-succinimidyl ether of Nα-carbobenzoxy-γ-tert-butylglutamine acid and pentafluorophenyl ether of N-tert-butyloxycarbonyl-L-threonine, and the nonapeptide is produced by condensation of a pentapeptide Z-Glu(OtBut)-Thr-Val-Glu(OtBut)-ArgOH with a tripeptide with a free amino group by the formula H-Glu(OtBut)-Lys(Boc)-Glu(OtBut)-OtBut followed by condensation with protected arginine.

EFFECT: method ensures production of a highly purified tetradecapeptide with an increased yield of the finished product.

5 cl, 3 dwg, 1 ex