METHOD FOR SEPARATING SULFATED GLYCOSOAMINEGLYCANES FROM BIOLOGICAL TISSUE Russian patent published in 2006 - IPC A61K35/12 

FIELD: medicine.

SUBSTANCE: method involves comminuting soft tissues to homogeneous state and hard tissues to small pieces, washing them with 0.1 N phosphate buffer. The tissue is digested in activated 0.125-0.325% papain at 60°C during 24 h, cooled during 10 h at 4°C, filtered and the digested tissue is poured into chromatography column where bone tissue collagen is used as solid carrier having particle sizes from 0.1 to 0.5 cm³, incubated during 10 to 24 h, washing in 0.05-0.2 N hydrochloric acid and eluted with 0.5-1.5N sodium chloride, dialyzed against saturated sodium chloride, precipitated in ethanol, centrifuged during 15 min at 1500 rpm at 4°C. The precipitate is washed with ethanol, dried, packed and sterilized using radiation method.

EFFECT: increased end product output; reduced antigenic activity; high biocompatibility.