METHOD FOR DETERMINING THE ENERGY METABOLISM ENZYME DYSFUNCTION IN RESPIRATORY PATHOLOGY IN CHILDREN AND APPLICATION THEREOF Russian patent published in 2021 - IPC A61B5/00 G01N33/68 G01N33/52 

FIELD: medicine.

SUBSTANCE: inventions relate to medicine, namely to clinical laboratory diagnostics, and can be used to determine the energy metabolism enzyme dysfunction in respiratory pathology in children. The activities of mitochondrial dehydrogenases and glycolysis in unaffected capillary blood lymphocytes are determined on a smear stained with nitroblue tetrazolium dye, subjected to microscopy. A morphological analysis of lymphocytes is conducted in each smear, and the amount and distribution of dye is determined in each cell. The activity of enzymes in lymphocytes is assessed by the determined amount of dye in conditional units. The conclusion about the presence of energy metabolism enzyme dysfunction is made by the values of the activities of succinate dehydrogenase and lactate dehydrogenase enzymes. The value of succinate dehydrogenase of 1 to 1.3 conditional units and the value of lactate dehydrogenase of 8 to 11 conditional units are taken as the norm. The activity of dehydrogenases can be determined by conducting quantitative morphological analysis of colour images using the computer program "Cell Composer". The method is used for executing control of the effectiveness of the assigned therapy, individually selecting medicinal products for pathogenetic therapy and correcting the therapy in time, as well as for the prognostic purposes in order to form risk groups for future development of allergic diseases.

EFFECT: method provides a possibility of creating an effective and informative method for clinical practice for diagnostics of cellular energy metabolism disorders and prognostic assessment of the course of the disease in respiratory pathology in children by determining the dysfunction of mitochondrial and glycolytic energy metabolism enzymes in lymphocytes from the capillary blood, as well as a possibility for application of the method for different clinical tasks.

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