FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology. The proposed method for the diagnosis of viruses and viral infections in vitro and in vivo. The method includes the use of accelerated mass spectrometry, while the quantitative calculation of the virus is carried out by the amount of radiocarbon label on the envelope of the viruses, where the label is introduced without destroying the virions. Samples for UMS analysis are prepared from a labeled vaccinated liquid with a known mass concentration by adding vaccinated liquid to carbon that does not contain radioactive 14С, carbonizing the resulting mixture and determining the content of the label in one viral particle.
EFFECT: counting ultra-low number of virions in vaccinated liquid and determination of the number of viral particles penetrated into any tissues infected with labeled viruses.
1 cl, 4 dwg, 3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF RECORDING SMALL AMOUNTS OF ORGANIC NANO- AND MICRO PARTICLES IN BIOLOGICAL TISSUES | 2016 |
|
RU2638820C1 |
| ADAPTED PANDEMIC INFLUENZA VIRUS STRAINS A/Tomsk/273/2010-MA1(H1N1pdm09), A/Tomsk/273/2010-MA2(H1N1pdm09) AND A/Tomsk/273/2010-MA3(H1N1pdm09) TO ASSESS THE EFFECT OF ANTIVIRAL DRUGS (VERSIONS) | 2015 |
|
RU2605317C1 |
| METHOD OF SAMPLE PREPARATION OF BIOORGANIC SAMPLES | 2014 |
|
RU2560066C1 |
| SAMPLING METHOD OF BIOORGANIC SAMPLES | 2015 |
|
RU2617364C1 |
| TEST SYSTEM FOR DIAGNOSTICS OF AGENTS OF ACUTE RESPIRATORY VIRAL INFECTIONS | 2019 |
|
RU2733379C1 |
| DIAGNOSTIC TECHNIQUE FOR AGENTS OF ACUTE RESPIRATORY VIRAL INFECTIONS | 2019 |
|
RU2726797C1 |
| STRAIN OF INFLUENZA VIRUS B/NOVOSIBIRSK/40/2017-MA FOR STUDYING THERAPEUTIC AND PREVENTIVE EFFICACY OF ANTIVIRAL PREPARATIONS IN VITRO AND IN VIVO | 2019 |
|
RU2703024C1 |
| N-SUBSTITUTED 1,4-DIAZABICYCLO-[2,2,2]-OCTANE DERIVATIVES EXHIBITING RNA-ANTIVIRAL ACTIVITY | 2008 |
|
RU2399669C2 |
| METHOD OF NEWCASTLE DISEASE PREVENTION | 2008 |
|
RU2403060C2 |
| METHOD OF OBTAINING LIVE CULTURAL ATTENUATED VACCINE FOR THE PREVENTION OF VARICELLA | 2016 |
|
RU2637093C1 |
