METHOD FOR DIAGNOSING DEGREE OF PERIODONTITIS BY DETERMINING PROTEINOLYTIC ACTIVITY OF ORAL FLUID MICROORGANISMS Russian patent published in 2022 - IPC G01N33/487 C12Q1/37 

FIELD: medicine.

SUBSTANCE: invention relates to medicine and relates to a method for diagnosing the degree of periodontitis by determining the proteinolytic activity of oral fluid microorganisms, characterized by that the oral fluid is collected on an empty stomach in a sterile bottle, saliva is introduced into centrifuge tubes, chloroform is added to the sample, centrifuged and the obtained supernatant is introduced into the wells with the prepared casein-containing culture medium, incubated, the proteolysis zones are developed by adding an aqueous solution of hydrochloric acid to the agar and the result is evaluated by the size of the proteolysis zones, while the size of the proteolysis zone of 5.3 ± 0.2 mm is considered to be a natural background of the opportunistic microbiota, 8.37 ± 0.3 mm - a mild degree of periodontitis, 13.3 ± 0.4 mm - a medium degree, 16.58 ± 0.2 mm - severe periodontitis.

EFFECT: invention provides diagnosing the degree of periodontitis.

1 cl, 1 dwg, 15 ex