FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and molecular biology and is a set of oligonucleotides for semi-quantitative evaluation of chicken DNA (Galus galus) in meat products by real-time PCR. The kit contains primers and a probe for detection of chicken species-specific DNA complementary to the Rarres1 gene region; primers and a probe for detecting mammalian and avian DNA complementary to a region of the ultraconserved genomic element VE1800. The kit can be used to calculate the content of chicken DNA (% DNA) relative to the total amount of mammalian and avian DNA in a sample.
EFFECT: using the invention, it is possible to differentiate falsification of meat products from trace amounts of undeclared chicken meat ingredients and from the presence of egg products.
1 cl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDES FOR DETECTING DNA OF GENETICALLY MODIFIED ATLANTIC SALMON BY REAL-TIME PCR METHOD | 2023 |
|
RU2808662C1 |
| SET OF SYNTHETIC OLIGONUCLEOTIDES FOR DETECTING DNAS OF REPRESENTATIVES OF DEER FAMILY | 2018 |
|
RU2722758C1 |
| METHOD FOR DETERMINING SPECIES IDENTITY OF TISSUES OF CHICKENS AND PIGS IN FOOD RAW MATERIALS, FODDER AND FOOD PRODUCTS | 2018 |
|
RU2700479C1 |
| TEST SYSTEM FOR DETERMINING SPECIES AFFILIATION OF TISSUES OF CHICKENS AND PIGS IN FOOD RAW MATERIALS, FODDER AND FOOD PRODUCTS | 2018 |
|
RU2700480C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF ANIMAL DNA IN FORAGE AND MEAT PRODUCTS | 2011 |
|
RU2560579C2 |
| METHOD FOR REAL-TIME PCR DETECTION OF TOXIGENIC PASTEURELLA MULTOCIDA | 2022 |
|
RU2785435C1 |
| METHOD FOR DETECTING DNA OF BOVINE HERPES VIRUS TYPE 6 IN BIOLOGICAL MATERIAL OF CATTLE BY REAL-TIME PCR | 2022 |
|
RU2787048C1 |
| METHOD OF IDENTIFYING DNA OF UREAPLASMA DIVERSUM BACTERIA IN BOVINE SEMEN BY REAL-TIME PCR | 2021 |
|
RU2770204C1 |
| SET OF OLIGONUCLEOTIDES FOR DETECTION OF RESISTANCE GENES TO TETRACYCLINES FROM THE tet GROUP IN BACTERIA BY PCR METHOD WITH REAL TIME DETECTION | 2022 |
|
RU2794156C1 |
| KIT OF SYNTHETIC OLIGONUCLEOTIDE SEQUENCES FOR IDENTIFICATION AND DETECTION OF QNRS AND QNRB GENES, PROVIDING RESISTANCE TO FLUOROQUINOLONES OF BACTERIA OF ENTEROBACTERIACEAE FAMILY, BY PCR METHOD WITH DETECTION IN "REAL TIME" MODE AND METHOD OF THEIR USE | 2022 |
|
RU2810576C1 |
