FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology and represents set of oligonucleotide primers for identification of DNA of animals, belonging to the group: pig, ox, hen, sheep, turkey, mouse, rat, dog, cat, and human DNA in dry or preserved forage for animals and in raw or processed by cooking meat products. Set contains first and second compositions of primers for identification of mitochondrial DNA, which provide possibility of simultaneous carrying out in similar temperature modes two multiprimer reactions of amplification by PCR method with increased specificity to identified mitochondrial DNA. To carry out multiprimer PCR reactions respectively first and second compositions of primers, where first composition includes primers belonging to the group SEQ ID NO: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 20, 21, and second composition includes primers, belonging to the group SEQ ID NO: 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21.
EFFECT: provision of animal DNA identification.
4 dwg, 8 tbl, 4 ex
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Authors
Dates
2015-08-20—Published
2011-05-12—Filed