IMMUNOLIPOSOME WHICH BINDS TO RBD OF S-PROTEIN SARS-CoV-2 Russian patent published in 2024 - IPC A61K39/395 

FIELD: biotechnology; medicine.

SUBSTANCE: described is an immunoliposome which binds to RBD of S-protein SARS-CoV-2, containing barnase in its inner volume, the outer surface of which is covalently modified with a monoclonal neutralizing antibody P4A1-2A. Also disclosed is a method of producing an immunoliposome, comprising: mixing a barnase solution with a phospholipid suspension, 7-fold freezing/thawing, forcing through an extruder using polycarbonate filters with a pore diameter of 100 nm, gel-permeable chromatography on a column with a sepharose sorbent CL-2B with subsequent modification for 40 minutes at room temperature of the outer surface of liposome with 2-iminothiolane to obtain a thiolated liposome, introducing a maleimide group into the P4A1-2A antibody by mixing with 100-fold molar excess of the sulfo-EMCS reagent and incubating for 40 minutes at room temperature, mixing the thiolated liposome containing barnase in the inner volume, with P4A1- 2A antibody containing maleimide groups, and incubation for 1 hour at room temperature to obtain an immunoliposome that binds to RBD of the S-protein SARS-CoV-2.

EFFECT: increased neutralizing activity of the P4A1-2A antibody.

4 cl, 6 dwg, 7 ex