METHOD FOR PRODUCING A HYPERIMMUNE SERUM AGAINST BOVINE ADENOVIRUS-10 IN CATTLE Russian patent published in 2022 - IPC A61K39/42 C07K16/08 C12N7/02 G01N33/531 

FIELD: veterinary medicine.

SUBSTANCE: invention relates to the field of chemistry and veterinary medicine, namely, to a method for producing a hyperimmune serum against Bovine-10 adenovirus of cattle, including the use of Bovine-10 culture adenovirus, purification thereof, production of an antigen and administration thereof to a rabbit, wherein a continuous culture of Taurus-1 (T-1), or Taurus-2 (T-2), or Taurus-4 (T-4) cells is used, infecting a dose of 0.1 to 0.3 TCD_50/cell, a virus is produced with an infectious titer of 6.0 to 7.0 lg TCD_50/ml, purified by means of hydrophobic and single-stage anion exchange chromatography, a suspension of the virus is produced with a protein concentration of 0.2 to 0.5 mg/ml, 0.2 ml of the virus suspension with complete Freund adjuvant is injected intracutaneously according to the plan on the 1st, 21st days at 20 points along the vertebral column, 0.2 ml of the virus suspension with incomplete Freund adjuvant is injected on the 35th, 50th, 80th days at 20 points along the vertebral column, and on the 110th, 130th days, 2.0 ml of the suspension without adjuvant is injected into the jugular vein, a hyperimmune serum with antibody titers is produced in a of neutralisation (PH) reaction not lower than 1:512 and in an indirect hemagglutination (IHA) reaction not lower than 1:1024.

EFFECT: production of a highly active and highly specific hyperimmune blood serum on laboratory animals immunised according to the plan with purified Bovine-10 adenovirus antigen.

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