FIELD: veterinary science.
SUBSTANCE: the present innovation deals with serological diagnostics of infectious diseases in cattle, viral diarrhea as mucosal disease in cattle (cattle VD) and evaluation of immunity strength in vaccinated animals. The method deals with growing finite cell line of coronary vessels in cow's embryo in Eagle's MEM nutritive medium with 20 mg/ml kanamycin and 10% equine serum. Double dilutions of inactivated tested serumal samples (56 C, 30 min) should be mixed at equal volume (0.05 ml) with 100 TCD50/0.1 ml virus to be incubated for 1 h at 37 C in CO2-incubator. On finishing the incubation one should add 0.1 ml suspension of the above-mentioned finite cell line in Eagle's MEM nutritive medium containing 20 mg/ml kanamycin and 5% (2.5% final concentration) of equine serum. Cell concentration in the culture corresponds to 3.5 x 105. Reaction registration should be carried out in 3 d while cytopathogenic viral action appears in control holes containing working viral dosage. The innovation enables to widen the number of diagnostic immunological methods for veterinary purposes.
EFFECT: higher efficiency of detection.
1 cl, 7 ex, 2 tbl
