FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, namely to a method for producing a bacteriocin-containing composition with antimicrobial activity. The cultivation of bacteria isolated from the soil is carried out at a temperature of 37°C on an agarized nutrient medium of the composition, g/l: bactopepton 10, ammonium citric acid 2, meat extract 10, sodium acetic acid 5, yeast extract 5, MgSO4⋅7H2O 0.1, glucose 20, MnSO4⋅5H2O 0.05, twin 80 1, Na2HPO4 2, or on an agarized nutrient medium composition, g/l: trypton 10, yeast extract 5, NaCl 10. The biomass is separated by centrifugation at 3900 rpm. Freeze-drying of the filler liquid is carried out at a vacuum of 0.05 mBar and a temperature of -20°C. The dry mass of the filler liquid is dissolved in 0.25 Μ phosphate buffer. The total protein is precipitated with ammonium sulfate. Proteins are divided into bacteriocins by gel-penetrating HPLC. Bacteriocins are purified on Amberlite XAD-2 resin. They are freeze-dried at a vacuum of 0.05 mBar and a temperature of -30°C. Bacteriocins are mixed in accordance with the recipes for obtaining a bacteriocin-containing composition.
EFFECT: invention makes it possible to expand the range of compositions containing bacteriocins with a wide spectrum of antimicrobial action for the treatment of diseases having a microbiological nature.
10 cl, 8 tbl, 2 ex
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