FIELD: medicine.
SUBSTANCE: invention relates to the field of medicine. The method is carried out by determining the content of biomarkers in the patient's oral fluid, including studies of the patient's oral fluid, while the patient's oral fluid is taken before or not earlier than 30 minutes after the patient has eaten, the patient's oral fluid is centrifuged at 3000 rpm for treatment 15 minutes with cooling to plus 5°C, dilution of the oral fluid with saline in a ratio of 1:100 and repeated centrifugation at 3000 rpm for 15 minutes with cooling to plus 5°C, placing the material of the patient’s oral fluid ready for examination into a cuvette and implementation of the enzyme immunoassay method on a plate based on monoclonal antibodies with subsequent analysis of the research results. The oral fluid is taken from the patient to study the formation of the mucous membrane of the retromolar region, tissue inhibitor of metalloproteinase 1 / tissue inhibitor of metalloproteinase 1 (TIMP 1 / TIMP 1) and tissue inhibitor of metalloproteinase 2 / tissue inhibitor of metalloproteinase 2 (TIMP 2 / TIMP) are chosen as biomarkers 2) and determine their quantitative content in the oral fluid of the patient. At the same time, the quantitative content of the biomarker in the oral fluid of the tissue inhibitor of metalloproteinase 1 / tissue inhibitor of metalloproteinase 1 (TIMP 1 / TIMP 1) of the clinical control group is taken as the level of 108.86-163.76 ng / ml, and the tissue inhibitor of metalloproteinase 2 / tissue inhibitor of metalloproteinase 2 (TIMP 2 / TIMP 2) clinical control groups take a level of 11.48-16.32 ng / ml, while if the content of tissue inhibitor of metalloproteinase 1 / tissue inhibitor of metalloproteinase 1 (TIMP 1 / TIMP 1) is determined in the oral fluid ) in the amount of 487.78-635.48 ng / ml and tissue inhibitor of metalloproteinase 2 / tissue inhibitor of metalloproteinase 2 (TIMP 2 / TIMP 2) in the amount of 53.01-70.25 ng / ml, then inflammation of the mucous membrane of the retromolar areas. At the same time, if the content of tissue inhibitor of metalloproteinase 1 / tissue inhibitor of metalloproteinase 1 (TIMP 1 / TIMP 1) is determined in the oral fluid in the amount of 244.67-417.65 ng / ml and tissue inhibitor of metalloproteinase 2 / tissue inhibitor of metalloproteinase 2 ( TIMP 2 / TIMP 2) in the amount of 22.92-41.74 ng / ml, then squamous cell carcinoma of the mucous membrane of the retromolar region is diagnosed when determining dental health, then, based on the results of determining the content of biomarkers in the oral fluid of the patient, the tactics of treating the patient are predicted.
EFFECT: method allows to increase the accuracy of differential express diagnostics of inflammation and squamous cell carcinoma of the mucous membrane of the retromolar region on the day of the patient's visit, to significantly increase the sensitivity of differential diagnosis of inflammation and squamous cell carcinoma of the mucous membrane of the retromolar region, to achieve a high probability of detecting the process of inflammation and squamous cell carcinoma of the mucous membrane of the retromolar region in the body of the patient both in the early stages of the disease, and at later stages, to significantly simplify the preparation of the patient for a certain time frame for the collection and storage of diagnostic material, in addition, there is no infection "through the blood" during the collection of diagnostic material, and there is also the possibility of taking diagnostic material from persons with poor venous filling (including the elderly and children).
3 cl, 2 ex
