VIRUS-LIKE CHIMERIC PARTICLES FOR INDUCTION OF SPECIFIC IMMUNITY AGAINST SEVERE ACUTE RESPIRATORY SYNDROME VIRUS SARS-COV-2 CONTAINING CORONAVIRUS AND ROTAVIRUS PROTEINS Russian patent published in 2022 - IPC A61K39/215 

FIELD: biotechnology and genetic engineering.

SUBSTANCE: inventions group relates to the field of biotechnology and genetic engineering. Disclosed is a virus-like chimeric particle containing one or more recombinant S proteins of various coronavirus strains having the nucleotide sequences of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, and recombinant proteins of rotavirus VP2 and VP6 having the nucleotide sequences SEQ ID NO:6, SEQ ID NO:7, which were obtained in the baculovirus insect expression system, while the virus-like particle mimics the SARS-CoV-2 virion. Also disclosed is a method for obtaining a virus-like chimeric particle, including obtaining recombinant baculoviruses containing genes for S proteins of various coronavirus strains and genes for rotavirus VP2 and VP6 proteins; co-infection of a continuous T.ni insect cell line with various combinations of recombinant baculoviruses; obtaining virus-like chimeric particles and their purification.

EFFECT: invention allows obtaining virus-like chimeric particles for induction of specific immunity against SARS-CoV-2 severe acute respiratory syndrome virus.

2 cl, 6 dwg, 6 ex