METHOD FOR DETERMINING THE PRESENCE OF A RESPONSE OF T CELLS IN HUMAN BLOOD TO THE PRESENCE OF SARS-CoV2 ANTIGENS Russian patent published in 2022 - IPC G01N33/48 

FIELD: medicine and biology.

SUBSTANCE: invention relates to medicine and biology, namely to immunological studies and studies of the functional activity of immune cells. Whole blood is taken in a volume of at least 2 ml into a test tube with an anticoagulant, then the resulting blood is distributed into several wells of the plate or tubes in a volume of at least 500 mcl each, then one well or tube is left as a control, phytohemagglutinin is added to the other at a concentration 10 mcg/ml, third - SARS-CoV2 antigen at a concentration of 20-30 mcg/ml, last - influenza A surface antigen at a concentration of 20-30 mcg/ml, after which the entire plate/all tubes are incubated for 24 hours, then the supernatant is taken in a volume of at least 150 mcl and a study of the level of interferon gamma is carried out, the results of which are used to judge the presence of a T-cell reaction to the presence of SARS-CoV2 antigens under the following conditions: the concentration of interferon gamma in the control well/tube is less than 100 pg/ml, the concentration of interferon gamma in the well/tube with phytohemagglutinin is more than 100 pg/ml, the concentration of interferon gamma in the well/test tube with SARS-CoV2 antigen is more than 20% higher than the concentration of interferon gamma in a well/tube with influenza A surface antigen.

EFFECT: invention provides the possibility of studying the reaction of immunocompetent whole blood cells to the presence of SARS-CoV2 antigens, which makes it possible to determine the specific response of T cells - the release of various cytokines - to the presence of SARS-CoV-2 antigens in the environment.

1 cl, 2 ex