METHOD FOR STUDYING MICROBIOTA OF LARGE INTESTINE BY POLYMERASE CHAIN REACTION METHOD WITH FLUORESCENCE DETECTION IN REAL TIME AND SET OF REAGENTS FOR IMPLEMENTATION THEREOF Russian patent published in 2022 - IPC C12Q1/68 

Abstract RU 2782316 C2

FIELD: biotechnology.

SUBSTANCE: decribed is a method for studying the microbiota of the large intestine by determining the DNA of intestinal microorganisms by the PCR method, including a microbiota sample interacting with a set of oligonucleotide primers, determining, for each oligonucleotide in said set of primers, the amount of nucleotide target sequences thereof present in said sample, and obtaining a microbiota profile from the relative amounts of nucleotide target sequences present in the sample. Also described is a corresponding set of primers.

EFFECT: invention expands the scope of tools for studying the intestinal microbiota.

2 cl

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RU 2 782 316 C2

Authors

Suvorova Mariia Aleksandrovna

Liudyno Viktoriia Iosifovna

Dates

2022-10-25Published

2020-12-07Filed