FIELD: biotechnology.
SUBSTANCE: decribed is a method for studying the microbiota of the large intestine by determining the DNA of intestinal microorganisms by the PCR method, including a microbiota sample interacting with a set of oligonucleotide primers, determining, for each oligonucleotide in said set of primers, the amount of nucleotide target sequences thereof present in said sample, and obtaining a microbiota profile from the relative amounts of nucleotide target sequences present in the sample. Also described is a corresponding set of primers.
EFFECT: invention expands the scope of tools for studying the intestinal microbiota.
2 cl
Title | Year | Author | Number |
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METHOD FOR STUDYING THE MICROBIOTA OF THE LARGE INTESTINE BY THE POLYMERASE CHAIN REACTION METHOD WITH FLUORESCENCE DETECTION IN REAL TIME AND SET OF REAGENTS FOR IMPLEMENTATION THEREOF | 2021 |
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Authors
Dates
2022-10-25—Published
2020-12-07—Filed