FIELD: microscopy.
SUBSTANCE: invention relates to light microscopy. Method for determining the basic focal plane includes determining the reference mark printed on the surface of a cover glass or the surface of a slide glass; scanning the cover glass or slide glass with an optical microscope in order to create an overview scanogram; locating the reference marks printed on the cover glass or slide glass based on the overview scanogram; focusing the optical microscope on the reference mark in order to calculate the focal distance of the reference mark; and calculating the basic focal plane determining the surface of the cover glass or the surface of the slide glass based on the focal length of the reference mark.
EFFECT: possibility of determining the basic focal plane in light microscopy.
41 cl, 28 dwg
| Title | Year | Author | Number |
|---|---|---|---|
| HOME TESTING DEVICE | 2014 |
|
RU2668332C1 |
| SYSTEM AND METHOD FOR ENHANCED PREDICTIVE AUTOFOCUSING | 2010 |
|
RU2522123C2 |
| MICROSCOPE COVER GLASS AND METHOD OF USING SAID COVER GLASS | 2006 |
|
RU2452986C2 |
| SYSTEM AND METHOD FOR MEASURING THE SURFACE OF SHAPED GLASS SHEETS | 2019 |
|
RU2790811C2 |
| LASER CENTRALIZER FOR X-RAY EMITTER | 2006 |
|
RU2325048C1 |
| METHOD FOR ANALYSIS OF ANALYTES WITH THE USE OF PARTICLES AS MARKS | 1997 |
|
RU2251572C2 |
| METHOD FOR FLUORESCENT HYBRIDIZATION IN SITU USING THE FGFR1 DNA PROBE IN DIFFERENT MAMMALIAN SPECIES ON CYTOLOGICAL PREPARATIONS | 2021 |
|
RU2755392C1 |
| DEVICE FOR APPLYING GRADIENT FORCES | 2001 |
|
RU2291511C2 |
| DEVICE FOR MONITORING DEFORMATIONS OF STRUCTURES AND FACILITIES OF LARGE AREA | 2017 |
|
RU2658110C1 |
| SYSTEM AND RELATED METHOD FOR DETECTING SMALL DEFECTS ON/IN GLASS SHEET ON PROCESS LINE | 2018 |
|
RU2763417C2 |
