METHOD FOR PRODUCTION OF HUMAN AND ANIMAL TISSUE MATRIX FOR USE AS GRAFTS, AUTOMATED METHOD FOR TREATMENT OF BIOLOGICAL TISSUE, RESERVOIR FOR THESE METHODS INCLUDED IN KIT Russian patent published in 2022 - IPC A61L27/36 B01L1/04 C12M1/00 C12M3/00 

FIELD: medicine.

SUBSTANCE: method for the production of tissue matrix for allografts or xenografts in an automated way inside one “classified” reaction reservoir is proposed, according to which, after collection of biological tissue, tissue is classified, cut, treated, and the resulting tissue matrix is packed. For treatment of tissue, a cycle of following stages is repeated: tissue is placed in a treatment solution until the end of treatment, tissue is treated in the specified solution with mixing, tissue is extracted from the treatment solution, the reaction reservoir is emptied. All stages of all treatment cycles are carried out inside one reaction reservoir by replacement of the treatment solution in the reaction reservoir between two cycles. The automated way for treatment of biological tissue for obtainment of tissue matrix for allografts or xenografts in the above-mentioned method contains a cycle of stages, at which tissue is placed to the treatment solution, tissue is treated in the specified solution with mixing, tissue is extracted from the treatment solution, the reaction reservoir is emptied, the specified stages are repeated until the end of treatment. All stages of all cycles are carried out in an automated way in a “classified” chamber and inside one reaction reservoir by replacement of the treatment solution in the reaction reservoir between two cycles. The reaction reservoir for use in the above-mentioned methods contains a container made with the possibility of accommodation of the treatment solution for biological tissue, in which a receiver for biological tissue and mixing means are placed. The receiver is made removable. A kit of components for single use for accompanying biological tissue in stages, to which tissue must be subjected before obtainment of tissue matrix, contains a tray, on which the above-mentioned reaction reservoir and a receiving socket for tissue to be treated are placed.

EFFECT: inventions provide an increase in the efficiency of automated tissue treatment by increasing performance, possibility of treatment of each tissue separately, and reduction in a number of reservoirs for treatment.

13 cl, 8 dwg