METHOD FOR PRODUCING MINERALIZED COMPOSITE MICROSCAFFOLDS FOR BONE TISSUE REGENERATION Russian patent published in 2018 - IPC A61K38/00 A61L27/58 

FIELD: pharmaceuticals.

SUBSTANCE: group of inventions relates to chemical-pharmaceutical industry and is a method for producing a mineralized composite microscaffold for bone tissue regeneration and the use of a mineralized microscaffold obtained by this method. Method comprises the steps of preparing an aqueous solution of silk fibroin, preparing an aqueous gelatin solution, forming a scaffold from a mixture of silk fibroin solutions and gelatin (7:3) with the addition of 1 % DMSO, cryocrushing of scaffold using a dispersant, sorting out the obtained microscaffold fragments and obtaining mineralized microscaffolds by immersing them in a solution containing 684 mM NaCl, 9.5 mM CaCl₂, 3.5 mM MgCl₂, 21 mM NaHCO₃ and 4 mM Na₂HPO₄, pH 4.0, and then immersing in a solution containing 684 mM NaCl, 9.5 mM CaCl₂, 0.7 mM MgCl₂, 10.5 mM NaHCO₃ and 4 mM Na₂HPO₄, pH 4.0 with further drying of the obtained product.

EFFECT: inventions allow to obtain mineralized composite microscaffolds with enhanced stimulation of osteogenesis in the absence of any inducers, as well as enhanced reorganization of the actin cytoskeleton, which is necessary for successful adhesion and differentiation of osteoblasts with simplification of technology.

2 cl, 1 tbl, 3 dwg, 3 ex